FLIPR Calcium Assay Kit
The FLIPR assay, commonly know to be the FLIPR Calcium Assay Kit allows the measurement of calcium levels in HEK-293 cells. It requires that cells be seeded in a confluent monolayer before being treated with the assay buffer. The seeding density depends on the type of cells and the culture conditions used. Since the expression levels of receptors change with cell passage number and drug selection, it is crucial to track changes in functional activity over time.
Fluo-4 NW (No-Wash) Calcium Assay Kit
The FLIPR assay and the Fluo-4 NW (No-Wash), calcium assay kit offers a proprietary calcium assay formulation that does not require the use of a quencher dye. This results in increased fluorescence intensity and lower variability. Additionally, no wash step is required, making this assay easier and faster.
The Fluo-4 NW calcium assay kit is suitable for use on adherent or microplates. It is designed for calcium measurements and is compatible with a wide range of cell types. It is also available in a HTS format.
Rhod-2 Calcium Flux Assay Kit
The Rhod-2 Calcium Flux Assay kit allows researchers to analyse intracellular calcium flux and quantify its levels. The dye itself is cell-permeable and fluoresces when there is a concentration of calcium in the cell. Its high emission wavelength makes it a good choice for intracellular calcium monitoring, flow cytometry, and confocal laser-scanning microscopy.
The kit comes with all the necessary reagents for performing ratiometric calcium flux assays. It is compatible with plate reader and fluorescence microscopy applications. The kit also includes individual components, giving users the flexibility to customise the assay to their specific needs.
Fluo-8 Calcium Flux Assay Kit
Fluo-8 Calcium Flux Assay Kit is a no-wash kit for detecting intracellular calcium mobilization in live cells. The kit uses Fluo-8, which is a fluorescent dye that can cross cell membranes and is cleaved by an esterase. As calcium binds to Fluo-8, its fluorescence increases.
Fluo-8 Calcium Flux Assay Kit is an effective tool for calcium measurements in drug discovery. It delivers a highly sensitive calcium measurement that is applicable to most cell culture systems. The kit also includes Carbachol to detect nitric oxide in cell culture medium.
Carbachol Dose Response in HEK-293 cells
The BD High Performance Calcium Assay Kit is a highly sensitive and specific assay that detects carbachol in HEK-293 cells. It has been designed for use in cell-based assays and can detect carbachol at concentrations up to 40 nM. This assay has a high signal to background ratio, and is ideal for rapid screening and lead discovery.
The Carbachol dose response in HEK-293 cells was investigated by comparing the response to a vehicle-induced stretch-activated calcium response. Carbachol (10-5 M) consistently induced a higher calcium signal when added to DMEM.